Methods of Studying Cells

Safe laboratory working practices

Before starting to work: Prepare or read an existing risk assessment with potential hazards of the experiment outlined. Report any damage to the equipment you are about to use and make sure you have all the personal protective equipment (PPE) required for the experiment.

Depending on the experiment's nature, PPE includes safety goggles, a laboratory coat, boots, face shields, and others.

When experimenting, follow the correct procedure.

Emergency procedures: In an emergency, you must be able to quickly locate emergency eyewash stations and showers, as well as a first aid kit. You will be supervised by your teacher or a lab technician who will advise you on these.

Good housekeeping: Avoid direct contact with potentially hazardous chemicals, do not eat or drink in the lab, wash your hands regularly, tie your hair back and use relevant PPE.

There may also be others; your teacher will guide you and read over the experiment carefully.

Cell biology lab techniques

Labs use many different techniques to study various cells, so let us take a closer look at some of them. First, we will briefly look at microscopy and how cell cultures work.

Cell microscopy

There are many different types of cell microscopy. The most notable are light microscopy and electron microscopy. Light microscopy is used to view tissues and cells, while electron microscopy is used to view cell organelles in detail.

Growing cell cultures using the streaking technique

The most common cell cultures are microbial cultures. These are done to detect bacteria in a sample or replicate a specific bacteria. The most well-known method for growing distinct bacterial colonies is the streaking technique. The working environment must be sterile, i.e. using an aseptic technique.

Equipment

• An isolated culture of bacteria
• Inoculation loop
• Bunsen burner (+ lighter/match to ignite)
• Agar plate (or any other sugar-based media suitable)

Procedure

1. Turn on your bunsen burner and turn the flame to a blue flame to start the process.
2. Sterilise the inoculation loop in the blue flame until it appears bright red. Let it cool.
3. Pick up some of the isolated colony with the loop and streak around 3 or 4 times in a parallel motion to cover about a quarter of your petri dish.
4. Put the loop under the flame again and when it cools, extend the streaks on the plate into the next quarter of the plate.
5. Repeat in the next two-quarters of the petri dish.

Techniques in cell and molecular biology

While cell biology looks at cells as a whole and their organelles, molecular biology looks at smaller parts within the cell, such as Chromosomes and DNA. Specific techniques are needed to find out more about these molecules. The main methods you should know are polymerase chain reactions (PCR) and gel electrophoresis.

Polymerase chain reaction (PCR)

This technique is used to copy small fragments of DNA so that there is enough DNA and can be further studied or used in tests that detect DNA, the Covid-19 PCR tests, for example.

Steps of PCR:

1. DNA denaturation - DNA sequence is denatured into single strands.
2. Annealing - DNA primers, nucleotides and Taq polymerase (heat stables) are added to the solution. DNA primers attach to the target nucleotide sequence.
3. Elongation - Taq polymerase binds to the DNA primers, and the strand is copied.
4. The cycle is repeated, and millions of DNA fragments are made.

Gel electrophoresis

Gel electrophoresis is used to separate molecules, including DNA, RNA and proteins, by molecular size. The molecules investigated are put in a gel that makes the molecules move using electrical currents. The gel contains small pores. The molecules differ in molecular sizes and will move at different speeds. Smaller molecules will generally travel faster than bigger ones because smaller molecules will pass through the pores in the gel more easily.

Gel electrophoresis allows scientists to distinguish between different lengths of DNA fragments.

Methods of studying eukaryotic cell structure

Cells can be studied in many different ways depending on the aspect to be studied. Eukaryotic cells are often larger than prokaryotic cells, making them easier to see. The most well-known method of studying eukaryotic cells is through the light microscope. The cell is usually put between two glass/clear plastic slides - the microscope slide and the coverslip. Cells can be stained to accentuate organelles of interest.

A light microscope can see bigger organelles such as the nucleus, cytoplasm, and cell wall.

Organelles of interest can be isolated by breaking open the cell and isolating the different organelles for commercial use. This process is called subcellular fractionation.

Prokaryotes, like bacteria, are often grown in a laboratory to be studied; they have high division rates. Eukaryotic cells can also be grown in cell cultures like prokaryotic cells. However, this is more complicated since eukaryotic cells do not replicate in the same way as prokaryotic cells do. They will take more time to replicate.